Characterization of liver fluke proteins for structure-based drug discovery
Our laboratory is working on the molecular and structural characterization of parasitic proteins of chemotherapeutic importance using state of the art techniques. These studies are intended to develop novel drugs/vaccines with therapeutic potential against the parasitic trematode- liver flukes. Apart from this, the work will contribute to a better understanding of the drug resistance mechanisms and novel insights into potential remedial approaches. Our long-term aspiration is to develop protein-based chemotherapeutic interventions to eradicate human sufferings, and develop therapeutics that are affordable to the developing world.
We use 2 different protein systems for our work.
1. The thiol based redox-active protein system.
2. Aminoacyl tRNA synthetases.
mRNA export and RNA Polymerase biology
The export of mRNA from the cell nucleus is one of the pillars of the gene expression pathway in eukaryotes. We approach basic questions in mRNA export by studying the major mRNA export factor, Mex67 in yeast and Tap/NXF1 in mammalian cells. To study the interactions of Mex67/Tap with other export factors and nuclear pore components, we use high-resolution imaging and biochemical and biophysical techniques.
RNAPII is thought to be central to
Role of solution variables in amyloidogenesis
Interest in amyloidogenesis has exploded in recent years, as scientists recognize the role of amyloid protein aggregates in neurodegenerative diseases such as Alzheimer's and Parkinson's disease. Assembly of proteins or peptides into mature amyloid fibrils is a multistep process initiated by conformational changes, during which intermediate aggregation states such as oligomers, protofibrils, and filaments are sampled. Because of its relevance to mechanism of disease, the paths traversed during fibrillogenesis, the kinetics of the process, and the solution variables affecting the fibrillation are of considerable interest. Even though significant advances have been made to understand the intermediates formed during fibrillogenesis, still a number of